74 PP
ANTIBACTERIAL ACTIVITY OF PROPOLIS PRODUCED IN BRAZIL AGAINST ACTINOBACILLUS ACTINOMYCETEMCOMITANS, FUSOBACTERIUM SPP. AND BACTERIA FROM THE BACTEROIDES FRAGILIS GROUP ISOLATED FROM HUMAN AND MARMOSET HOSTS

Santos, F.A.*1,2, Bastos, E.M.A.F 3., Uzeda, M.1, Carvalho, M.A.R.2, Farias, L..M.2 and Moreira, E.S.A.2,

Department of Microbiology, Universidade Federal de Minas Gerais2, Fundação Ezequiel Dias - MG3 and Universidade Federal do Rio de Janeiro1, Rio de Janeiro, Brazil.

The aim of this study was to assay the inhibitory effect of ethanolic extract of propolis (EEP) against Actinobacillus actinomycetemcomitans (14 from human oral cavity, 14 from marmoset oral cavity and two reference strains), Fusobacterium spp.,(4 from human oral cavity, 4 from marmoset oral cavity and two reference strains), and against bacteria from the Bateroides fragilis Group isolated from human (5) and marmoset (6) stools and also against reference strains (11). Four commercial EEPs samples (CSI,CSII,CSIII and CSIV) and 8 samples collected during the four seasons (2 in the summer, 2 in the autumn, 2 in the winter and 2 in the spring) from an experimental apiary located in a Cerrado Vegetation in Brazil were used. The EEP antibacterial activity was assayed by the method of dilution in agar with serial concentrations ranging from 0.025% to 2.0% (V/V%). It was observed that the 30 A. actinomycetemcomitans strains were inhibited in the concentrations of 0,1% to 0,25%, and did not grow at all at 0,5%. Inhibition of the 10 Fusobacterium spp. was observed at concentrations of 0,05% to 0,1%, and no growth was observed at 0,25%. The 22 Bacteroides from the B. fragilis Group were inhibited at 0.1% to 0.25% and no growth was observed at 0.5%. Among all the bacteria tested the Fusobacterium were the most susceptible one and, among the commercial EEPs, the sample CSIV demonstrated a higher inhibitory effect. It was observed that the EEPs inhibited bacterial growth efficiently at low concentrations. With exception to the tests with A. actinomycetemcomitans strains, no significant difference was detected with regards to the effect of seasonality and the inhibitory activity of propolis.

Supported by FAPEMIG and CNPq.